Microscope Kinds

MICROSCOPES

Bright field microscopy is the simplest optical microscopy illumination  (i.e., illuminated from below and observed from above) using white light; the typical appearance is a dark sample on a bright background.(RP=200nm or 0.2 um)

Dark field microscopy (dark ground microscopy)  the specimen appears bright against a dark background

Phase contrast microscopy converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations.

Fluorescence microscope uses fluorescence and phosphorescence in addition to, reflection and absorption to study properties of organic or inorganic substances as well as microorganisms .(RP=100nm or 0.1 um)

UV microscope uses UV radiation and is designed to image in regions beyond the visible range; twice the magnifying power of light microscope. .(RP=100nm or 0.1 um)

Scanning electron microscope (SEM) is a type of electron microscope that produces (surface) images of a sample by scanning it with a focused beam of electrons. SEM can achieve resolution better than 1 nanometer. Specimens can be observed onto an imaging device. 

Transmission electron microscopy (TEM) is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through. An image is formed from the interaction of the electrons transmitted through the specimen; the image is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera.

(RP= 0.2nm)